Bypassing the Requirement for an Essential MYST Acetyltransferase

Bypassing the requirement for an essential MYST acetyltransferase.

Histone acetylation is a key regulatory feature for chromatin that is established by opposing enzymatic activities of lysine acetyltransferases (KATs/HATs) and deacetylases (KDACs/HDACs). Esa1, like its human homolog Tip60, is an essential MYST family enzyme that acetylates histones H4 and H2A and other nonhistone substrates. Here we report that the essential requirement for ESA1 in Saccharomyc...

MYST 4 ( MYST histone acetyltransferase ( monocytic leukemia ) 4 )

Autoacetylation of the MYST Lysine Acetyltransferase MOF

The MYST family of histone acetyltransferases (HATs) plays critical roles in diverse cellular processes, such as the epigenetic regulation of gene expression. Lysine autoacetylation of the MYST HATs has received considerable attention recently. Nonetheless, the mechanism and function of the autoacetylation process are not well defined. To better understand the biochemical mechanism of MYST auto...

Catalytic-site mutations in the MYST family histone Acetyltransferase Esa1.

Esa1 is the only essential histone acetyltransferase (HAT) in budding yeast. It is the catalytic subunit of at least two multiprotein complexes, NuA4 and Piccolo NuA4 (picNuA4), and its essential function is believed to be its catalytic HAT activity. To examine the role of Esa1 in DNA damage repair, we isolated viable esa1 mutants with a range of hypersensitivities to the toposide camptothecin....

Telemedicine: an Essential Requirement for the Health Care Providers, with Emphasis on Legal Aspects

Telemedicine is the use of telecommunication and information technologies in order to provide clinical health care at a distance. These technologies allow communications between patient and medical staff with convenience as well as the transmission of medical, imaging and health informatics data from one site to another. It is also used to save lives in critical care and emergency situations. A...